Chelsea have been denied a request to freeze a transfer ban while they appeal against the punishment handed out by world soccer governing body, Fifa, reports BBC sport.
The Blues have been banned from registering new players until January 2020 for breaking rules over the signing of foreign under-18 players.
Chelsea deny any wrongdoing and have appealed against the decision.
But Fifa says it has denied measures which would see the transfer ban frozen during the appeal process.
The Blues have also been fined £460,000, while the Football Association has been fined £390,000.
In previous cases, involving Spanish clubs, Fifa has relaxed the measures such that transfer bans were not enforced during the appeal process.
The ban, which covers two transfer windows, does not prevent the release of players and will not apply to the club’s women’s and futsal teams.